JoVE Journal

Bioengineering

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Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy

3.4K Views

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06:37 min

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June 15th, 2022

DOI :

10.3791/63931-v

June 15th, 2022

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Hannah N. Verwei¹, Gloria Lee², Gregor Leech², Irene Istúriz Petitjean³, Gijsje H. Koenderink³, Rae M. Robertson-Anderson², Ryan James McGorty²

¹Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, ²Department of Physics and Biophysics, University of San Diego, ³Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology

Summary

Differential dynamic microscopy (DDM) combines features of dynamic light scattering and microscopy. Here, the process of using DDM to characterize reconstituted cytoskeleton networks by quantifying the subdiffusive and caged dynamics of particles in vimentin networks and the ballistic motion of active myosin-driven actin-microtubule composites is presented.

Explore More Videos

Cytoskeleton Dynamics

Differential Dynamic Microscopy

Optical Microscopy

Software Package

Reconstituted Networks

Image Sequences

PyDDM Code Repository

Fitting Parameters

Metadata Section

Analysis Parameters

Background Estimation

Dynamic Quantification

Chapters in this video

0:04

Introduction

0:45

Image Acquisition and Parameter Setup

1:55

Calculating the DDM Matrix

2:54

Fitting the DDM Matrix or the ISF

3:38

Interpreting the Fit Results

4:26

Results: DDM Analysis for Vimentin and Actin-Microtubule Composite Network

6:09

Conclusion

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