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Bioengineering

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Nanyang Technological University

Formulating and Characterizing an Exosome-based Dopamine Carrier System

2.9K Views

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06:08 min

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April 4th, 2022

DOI :

10.3791/63624-v

April 4th, 2022

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Burçak Yavuz¹^,², Hakan Darici³^,⁴^,⁵^,⁶, Aslı Pınar Zorba Yildiz²^,⁷, Emrah Şefik Abamor², Murat Topuzoğullari², Melahat Bağirova⁸, Adil Allahverdiyev⁸, Erdal Karaoz³^,⁴^,⁵^,⁶^,⁹

¹Vocational School of Health Services/İstanbul, Altinbas University, ²Chemical Metallurgy Faculty, Department of Bioengineering, Yildiz Technical University, ³Faculty of Medicine, Department of Histology & Embryology, Istinye University, ⁴3D Bioprinting Design & Prototyping R&D Center, Istinye University, ⁵Stem Cell and Tissue Engineering R&D Center, Istinye University, ⁶Medicine Faculty, Istinye University, ⁷Vocational School of Health Services, Istinye University, ⁸The V. Akhundov National Scientific Research Medical Prophylactic Institute, ⁹Center for Stem Cells and Regenerative Medicine (LivMedCell), Liv Hospital

Summary

Here, we aimed to obtain a formulation by dopamine loading of the isolated exosomes of stem cells from Wharton's jelly mesenchymal stem cells. Exosome isolation and characterization, drug loading into the resulting exosomes, and the cytotoxic activity of the developed formulation are described in this protocol.

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Dopamine Carrier System

Extracellular Vesicles

Central Nervous System

Blood brain Barrier

Mesenchymal Stem Cells

Wharton s Jelly

Immunogenic Potential

Dopamine Solution

Chapters in this video

0:04

Introduction

0:56

Culturing and Cryopreservation of Wharton's Jelly Mesenchymal Stem Cells

1:30

Production of Exosomes from Wharton's Jelly Mesenchymal Stem Cells

2:20

Dopamine Loading into Exosomes and Characterization of Dopamine Loaded Exosomes

3:15

Results: Development of a Nanocarrier System by Encapsulation of Dopamine into Exosomes Isolated from WJ‐MSCs

5:07

Conclusion

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