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Nanyang Technological University

Application of Passive Head Motion to Generate Defined Accelerations at the Heads of Rodents

1.7K Views

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05:04 min

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July 21st, 2022

DOI :

10.3791/63100-v

July 21st, 2022

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Takahiro Maekawa*¹, Naoyoshi Sakitani*¹, Youngjae Ryu¹, Atsushi Takashima², Shuhei Murase¹, Julius Fink³, Motoshi Nagao¹, Toru Ogata⁴, Masahiro Shinohara¹, Yasuhiro Sawada⁵

¹Department of Rehabilitation for Motor Functions, National Rehabilitation Center for Persons with Disabilities, ²Department of Assistive Technology, National Rehabilitation Center for Persons with Disabilities, ³Department of Metabolism and Endocrinology, Graduate School of Medicine, Juntendo University, ⁴Department of Rehabilitation Medicine, Graduate School of Medicine, The University of Tokyo, ⁵Department of Clinical Research, National Rehabilitation Center for Persons with Disabilities

Summary

The present protocol describes a custom-designed ''passive head motion'' system, which reproduces mechanical accelerations at rodents' heads generated during their treadmill running at moderate velocities. It allows dissecting mechanical factors/elements from the beneficial effects of physical exercise.

Explore More Videos

Passive Head Motion

Defined Accelerations

Physical Exercise

Organism Homeostasis

Rat Treadmill Experiment

Accelerometer Measurement

Vertical Accelerations

Amplitude Of Oscillation

Hydroxytryptophan Administration

Mouse Brain Analysis

Primary Antibody Application

Fluorescent Secondary Antibody

Wet Mounting Technique

Chapters in this video

0:05

Introduction

0:35

Measurement of Magnitudes of Accelerations and Adjustment of the PHM System

1:33

Quantification of Mouse Head-Twitch Response

2:03

Immunohistochemical Analysis of Prefrontal Cortex (PFC) Collected from Euthanized Mouse Brain

3:22

Results: Effect of Treadmill Running and PHM Application in Mouse PFC Neurons

4:37

Conclusion

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