JoVE Journal

Bioengineering

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Nanyang Technological University

Improving Reproducibility to Meet Minimal Information for Studies of Extracellular Vesicles 2018 Guidelines in Nanoparticle Tracking Analysis

Nanoparticle tracking analysis (NTA) is a widely used method to characterize extracellular vesicles. This paper highlights NTA experimental parameters and controls plus a uniform method of analysis and characterization of samples and diluents necessary to supplement the guidelines proposed by MISEV2018 and EV-TRACK for reproducibility between laboratories.

Chapters in this video

0:04

Introduction

0:44

Cleaning and Assembly of the Laser Module

1:29

Flushing Procedure for the Laser Module Prior to and Between Samples

2:28

Focusing and Positioning of the Laser Module

3:34

Loading Standards/Samples/Diluent into the Laser Module for Nanoparticle Tracking Analysis (NTA)

4:28

Sample Nanoparticle Tracking Analysis (NTA)

6:06

Re-Analysis of the Current Sample at Different Detection Threshold

6:40

Results: Effects of Camera Level and Detection Threshold on Measured Particle Size and Concentration of Filtered and Unfiltered Samples

8:02

Conclusion

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