Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632
4.9K Views
•
06:47 min
•
November 6th, 2021
DOI :
November 6th, 2021
•Zhiwei Xie1,2,3, Jizhou Shi4, Min Zong3, Qiuping Xu1, Chang Liu1, Jie Wen1, Qun Zhang1, Panpan Liu1, Guanyi Liu1, Jing Guo2,5, Xunwei Wu1
1Department of Tissue Engineering and Regeneration, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 2Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 3Department of Stomatology, Shengli Oilfield Central Hospital, 4Department of Pediatric Surgery, Shengli Oilfield Central Hospital, 5Ningbo Stomatology Hospital of Savaid Stomatology School, Hangzhou Medical College
Chapters in this video
0:00
Introduction
0:49
Gingival Tissue Pretreatment
1:13
Digestion of Gingival Tissue
2:46
Cell Passage
3:51
Representative Results
6:18
Conclusion
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