Generation of Human Motor Units with Functional Neuromuscular Junctions in Microfluidic Devices

4.5K Views

•

10:48 min

•

September 7th, 2021

DOI :

10.3791/62959-v

September 7th, 2021

•

Katarina Stoklund Dittlau¹^,², Emily N. Krasnow¹^,², Laura Fumagalli¹^,², Tijs Vandoorne¹^,², Pieter Baatsen³^,⁴, Axelle Kerstens³^,⁴, Giorgia Giacomazzi⁵, Benjamin Pavie³^,⁴, Elisabeth Rossaert¹^,², Jimmy Beckers¹^,², Maurilio Sampaolesi⁵, Philip Van Damme¹^,²^,⁶, Ludo Van Den Bosch¹^,²

¹Department of Neurosciences, Experimental Neurology, and Leuven Brain Institute, KU Leuven - University of Leuven, ²VIB Bio Imaging Core, KU Leuven - University of Leuven, ³Department of Development and Regeneration, Stem Cell and Developmental Biology, KU Leuven - University of Leuven, ⁴Department of Neurology, University Hospitals Leuven

Summary

Explore More Videos

Human Motor Units

Neuromuscular Junctions

Microfluidic Devices

Motor Neurons

Amyotrophic Lateral Sclerosis

Chapters in this video

0:05

Introduction

0:54

Pre-Assembled Microfluidic Device Preparation

2:39

Silicone Microfluidic Device Preparation

5:18

Neural Progenitor Cell Plating in Microfluidic Devices

7:15

Human Primary Mesoangioblast (MAB) Plating in Microfluidic Devices

8:32

Results: Generation of NMJs in Microfluidic Devices

10:10

Conclusion

Related Videos

12:18

The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy

21.5K Views

08:01

Analysis of Developing Tooth Germ Innervation Using Microfluidic Co-culture Devices

8.2K Views

10:41

Why Quantification Matters: Characterization of Phenotypes at the Drosophila Larval Neuromuscular Junction

8.1K Views

10:26

Rewiring Neuronal Circuits: A New Method for Fast Neurite Extension and Functional Neuronal Connection

8.7K Views

06:42

Conducting Hyperscanning Experiments with Functional Near-Infrared Spectroscopy

9.7K Views

08:20

3D Kinematic Analysis for the Functional Evaluation in the Rat Model of Sciatic Nerve Crush Injury

8.5K Views

10:12

Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System

9.9K Views

09:57

Three-Dimensional Motor Nerve Organoid Generation

9.0K Views

06:54

Functional Isolation of Single Motor Units of Rat Medial Gastrocnemius Muscle

5.2K Views

08:41

Dissection of Single Skeletal Muscle Fibers for Immunofluorescent and Morphometric Analyses of Whole-Mount Neuromuscular Junctions

8.2K Views