JoVE Journal

Bioengineering

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Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System (MMC)

3.5K Views

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08:18 min

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February 18th, 2022

DOI :

10.3791/62800-v

February 18th, 2022

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Xingjin Jian*¹^,², Xiaojie Guo*¹^,²^,³, Jia Wang⁴, Zheng Lin Tan¹^,²^,⁶, Xin-hui Xing¹^,²^,⁵, Liyan Wang³, Chong Zhang¹^,²^,⁵

¹Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University, ²Key Laboratory of Industrial Biocatalysis, Ministry of Education, Tsinghua University, ³Luoyang TMAXTREE Biotechnology Co., Ltd., ⁴Biochemical Engineering Research Group, School of Chemical Engineering and Technology, Xi’an Jiaotong University, ⁵Center for Synthetic & Systems Biology, Tsinghua University, ⁶School of Life Science and Technology, Tokyo Institute of Technology

Summary

This protocol describes how to use the Microbial Microdroplet Culture system (MMC) to conduct automated microbial cultivation and adaptive evolution. MMC can cultivate and sub-cultivate microorganisms automatically and continuously and monitor online their growth with relatively high throughput and good parallelization, reducing labor and reagent consumption.

Explore More Videos

Automated Microbial Cultivation

Microbial Microdroplet Culture System

Adaptive Evolution

Microbiology Research

Growth Curve Measurement

Laboratory Evolution

Microfluidic Chip

Escherichia Coli MG1655

Initialization Process

Cultivation Temperature

Photoelectric Signal Value

Sterile Syringe

Chapters in this video

0:04

Introduction

0:51

Preparations for the Experiment

1:48

Growth Curve Measurement in Microbial Microdroplet Culture System (MMC)

4:25

Adaptive Evolution in Microbial Microdroplet Culture System (MMC)

6:41

Results: The Adaptive Evolution of the Methanol-Essential Escherichia coli (MeSV2.2) in MMC

7:36

Conclusion

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