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Biology
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Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
4.5K Views
•
07:54 min
•
March 31st, 2021
DOI :
March 31st, 2021
•Raphael Nyaruaba1,2,3, Xiaohong Li1, Caroline Mwaliko2,3,4, Changchang Li1, Matilu Mwau5, Nelson Odiwour1,2,3, Elishiba Muturi1,2,3, Caroline Muema1,2,3, Junhua Li1, Junping Yu1, Hongping Wei1
1Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, 2International College, University of Chinese Academy of Sciences, 3Sino-Africa Joint Research Center, 4CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 5Center for Infectious and Parasitic Diseases Control Research, Kenya Medical Research Institute
Chapters in this video
0:04
Introduction
0:48
cDNA and Droplet Digital Polymerase Chain Reaction (ddPCR) Preparation
2:03
Automated Droplet Generation
2:59
PCR Amplification and Droplet Reading
4:09
Data Analysis
6:01
Results: SARS‐CoV‐2 Detection Using a Two‐Color ddPCR System
7:07
Conclusion
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