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Nanyang Technological University

Microdissection of Mouse Brain into Functionally and Anatomically Different Regions

44.1K Views

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08:06 min

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February 15th, 2021

DOI :

10.3791/61941-v

February 15th, 2021

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James Meyerhoff¹, Seid Muhie¹, Nabarun Chakraborty², Lalith Naidu³, Bintu Sowe³, Rasha Hammamieh², Marti Jett⁴, Aarti Gautam²

¹The Geneva Foundation, Medical Readiness Systems Biology, Walter Reed Army Institute of Research, ²Walter Reed Army Institute of Research, Medical Readiness Systems Biology, Center for Military Psychiatry and Neuroscience, ³ORISE Fellow, Medical Readiness Systems Biology, Walter Reed Army Institute of Research, ⁴Headquarter, Walter Reed Army Institute of Research

Summary

We present a hands-on, step-by-step, rapid protocol for mouse brain removal and dissection of discrete regions from fresh brain tissue. Obtaining brain regions for molecular analysis has become routine in many neuroscience labs. These brain regions are immediately frozen to obtain high quality transcriptomic data for system level analysis.

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Microdissection

Anatomical Complexity

Molecular Integrity

Dissection Protocol

Structural Landmarks

Bioinformatician

Surgical Procedure

Meninges Separation

Cerebellum Exposure

Parasagittal Cut

Chapters in this video

0:04

Introduction

1:41

Mouse Brain Removal

3:26

Mouse Brain Dissection

6:40

Results: Mouse Brain Removal and Dissection

7:22

Conclusion

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