A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice
7.2K Views
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09:17 min
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January 5th, 2021
DOI :
January 5th, 2021
•Sonia Simonetti*1,2, Ambra Natalini*1,2, Giovanna Peruzzi3, Alfredo Nicosia4, Antonella Folgori5, Stefania Capone5, Angela Santoni2,6, Francesca Di Rosa1
1Institute of Molecular Biology and Pathology, National Research Council of Italy (CNR), 2Department of Molecular Medicine, University of Rome “Sapienza”, 3Center for Life Nano Science, Istituto Italiano di Tecnologia, 4Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 5Reithera Srl, 6IRCCS, Neuromed
Chapters in this video
0:04
Introduction
0:57
Intracellular Staining
2:41
Data Analysis
7:16
Results: Cell Cycle Analysis of BM Cells and Antigen-Specific CD8 T Cells form LNs and Spleen
8:30
Conclusion
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