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Nanyang Technological University

Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia

4.1K Views

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05:24 min

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February 21st, 2021

DOI :

10.3791/61803-v

February 21st, 2021

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Henrik Landerer*¹, Marlon Arnone*¹, Ronja Wieboldt², Elsa Goersch¹, Anna M. Paczulla Stanger³, Martina Konantz¹, Claudia Lengerke¹^,²^,³

¹Department of Biomedicine, University Hospital Basel and University of Basel, ²Division for Hematology, University Hospital Basel and University of Basel, ³Department of Internal Medicine, Hematology and Oncology, University Hospital Tübingen

Summary

We present two different staining protocols for NKG2D ligand (NKG2DL) detection in human primary acute myeloid leukemia (AML) samples. The first approach is based on a fusion protein, able to recognize all known and potentially yet unknown ligands, while the second protocol relies on the addition of multiple anti-NKG2DL antibodies.

Explore More Videos

Cytometric Approaches

Surface Detection

Acute Myeloid Leukemia

NKG2D Fusion Protein

Staining Method

Leukemic Stem Cells

Streptavidin PE

Chapters in this video

0:04

Introduction

0:43

Biotinylation of the NKG2D Fusion Protein and Thawing of Primary AML Cells

2:00

Staining of Primary AML Cells with the Biotinylated NKG2D Fusion Protein

3:24

Results: Gating Strategy and Staining Protocol Comparison

4:49

Conclusion

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