Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)

13.2K Views

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10:22 min

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June 30th, 2020

DOI :

10.3791/61440-v

June 30th, 2020

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Inés Medina-Lozano*¹^,⁴, Juan Ramón Bertolín*²^,⁴, Raquel Zufiaurre³, Aurora Díaz¹^,⁴

¹Unidad de Hortofruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), ²Unidad de Producción y Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), ³Dpto Química Analítica, Escuela Politécnica Superior (Universidad de Zaragoza), ⁴Instituto Agroalimentario de Aragón - IA2 (CITA-Universidad de Zaragoza)

Summary

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UPLC UV Method

Vitamin C Quantification

Chapters in this video

0:04

Introduction

1:03

Plant Material Preparation

1:57

Ascorbic Acid (AA) Standard Preparation

2:34

AA and Dehydroascorbic Acid (DHAA) Extraction

3:40

DHAA Reduction for Total AA (TAA) Extraction

4:31

Ultra-High-Performance Liquid Chromatography-Ultraviolet Preparation

6:46

AA Determination

7:21

AA and DHAA Quantification

8:18

Results: Representative Vitamin C Quantification in Lactuca Species

9:44

Conclusion

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