Worm-align and Worm_CP, Two Open-Source Pipelines for Straightening and Quantification of Fluorescence Image Data Obtained from Caenorhabditis elegans
6.0K Views
•
11:16 min
•
May 28th, 2020
DOI :
May 28th, 2020
•Chapters in this video
0:05
Introduction
0:57
Mounting Fixed Worms for Imaging
2:56
Creating Montage Images of Aligned Single Worms Using the Worm-align FIJI Pipeline
6:01
Analyzing Single-worm Fluorescence Intensity Using the Output from Worm-align in an Automated CellProfiler Pipeline
8:07
Results: Fluorescence Quantification with the Worm_CP Pipeline
9:50
Conclusion
Related Videos
09:23
Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans
8.0K Views
08:01
Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton
6.2K Views
06:50
Calcium Imaging in Freely Behaving Caenorhabditis elegans with Well-Controlled, Nonlocalized Vibration
2.6K Views
07:51
Agarose Microchambers for Long-term Calcium Imaging of Caenorhabditis elegans
9.5K Views
09:00
A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans
6.4K Views
08:47
Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans
9.6K Views
07:50
Automating Aggregate Quantification in Caenorhabditis elegans
2.7K Views
09:54
Using Single-Worm Data to Quantify Heterogeneity in Caenorhabditis elegans-Bacterial Interactions
2.1K Views
11:59
Detection of Mitophagy in Caenorhabditis elegans and Mammalian Cells Using Organelle-Specific Dyes
2.1K Views
05:47
Optimizing Visualization of Axonal Transport of Endogenous Cargo by Fluorescence Microscopy in Living Caenorhabditis elegans
662 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved