Simultaneous Quantification of Selected Kynurenines Analyzed by Liquid Chromatography-Mass Spectrometry in Medium Collected from Cancer Cell Cultures
3.3K Views
•
10:38 min
•
May 9th, 2020
DOI :
May 9th, 2020
•Chapters in this video
0:04
Introduction
0:57
3-Nitrotyrosine (3NT), Kynurenine (Kyn), 3-Hydroxykynurenine (3HKyn), 3-Hydroxyanthranilic Acid (3HAA), Xanthurenic Acid (XA) and Charcoal Treated Culture Medium Preparation
1:58
Calibration Solution Preparation
3:42
Liquid Chromatography-Mass Spectrometry (LC-MS) System Setu
4:20
Calibration Curve Construction
4:55
Sample Collection
5:33
Sample Preparation for LC-MS Analysis
6:30
Protein Content Assessment and Data Normalization
8:12
Results: Representative LC-MS Data of Selected KYNURENINES Determination
9:57
Conclusion
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