Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ

1.9K Views

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10:05 min

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May 8th, 2020

DOI :

10.3791/60859-v

May 8th, 2020

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Jesús Espada¹^,², Elisa Carrasco³, María I. Calvo-Sánchez¹^,⁴, Sandra Fernández-Martos¹, Juan José Montoya⁵

¹Ramón y Cajal Institute for Health Research (IRYCIS), Ramón y Cajal University Hospital, ²Centro Integrativo de Biología y Química Aplicada (CIBQA), Universidad Bernardo O'Higgins, ³Department of Molecular Biology, Faculty of Sciences, Universidad Autónoma de Madrid (UAM), ⁴Instituto de Investigaciones Biosanitarias, Facultad de Ciencias Experimentales, Universidad Francisco de Vitoria, ⁵Department of Radiology, Rehabilitation & Physiotherapy, Faculty of Medicine, Complutense University

Summary

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Stem Cells

Tissue Regeneration

Reactive Oxygen Species

Experimental Methodology

User friendly

Non invasive Procedure

Chapters in this video

0:04

Introduction

1:26

Hair Growth and Burn Induction and Long‐Term 5‐bromo‐2 -deoxyuridine (BrdU) Label Retaining Cell (LRC) Generation

2:58

Transient Nonlethal Reactive Oxygen Species (ROS) Production

4:37

BrdU Detection in Mouse Tail Skin

5:38

ROS Detection in Mouse Tail Skin

7:16

Results: Effects of Photodynamic mALA Treatment and Transient ROS Production

9:00

Conclusion

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