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Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System

6.8K Views

09:12 min

March 10th, 2020

DOI :

10.3791/60693-v

March 10th, 2020


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Live Cell Imaging

Chapters in this video

0:04

Introduction

0:55

Live Cell Imaging of L. pneumophila with Fluorescently Tagged Dot/Icm Components

2:47

Quantification of Polar Localization and Dynamics of Dot/Icm Components

5:36

Detection of sfGFP Mass Density with Cryo-ET

6:23

Results: Imaging Dot/Icm Secretion Systems in Intact Bacteria

8:17

Conclusion

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