Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries

9.0K Views

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08:49 min

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January 22nd, 2019

DOI :

10.3791/58946-v

January 22nd, 2019

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Elijah W. Chen*¹, Chyan Ying Ke*²^,³, Joanna Brzostek*¹, Nicholas R. J. Gascoigne¹, Vasily Rybakin¹^,⁴

¹Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, ²Singapore Immunology Network, A*STAR, ³Curiox Biosystems, ⁴Department of Immunobiology, Rega Institute for Medical Research, Katholieke Universiteit (KU) Leuven

Summary

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T cell Receptor Signaling

Chemical Inhibitor Libraries

High throughput Assay

TCR Signaling Modulators

Thymocyte Stimulation

Kinase Inhibitors

Immune Diseases Therapy

Chapters in this video

0:04

Title

1:15

Kinase Inhibitor Library Screening (Centrifuge-independent Assay)

2:15

Stimulation of Thymocytes Using Anti-CD3/CD28 Beads

3:52

Staining of Surface Antigens and Fixation of Cells

5:09

Intracellular Staining for Active Caspase 3

6:05

Results: Flow-Cytometry-Based Screening of Modulators of T-cell Receptor Signaling and T-cell Activation

7:59

Conclusion

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