JoVE Journal

Immunology and Infection

This content is Open Access.

Automated Behavioral Analysis of Large C. elegans Populations Using a Wide Field-of-view Tracking Platform

9.1K Views

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07:20 min

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November 28th, 2018

DOI :

10.3791/58643-v

November 28th, 2018

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Michele Perni¹, Sam Casford¹, Francesco A. Aprile¹, Ellen A. Nollen², Tuomas P.J. Knowles¹, Michele Vendruscolo¹, Christopher M. Dobson¹

¹Centre for Misfolding Diseases, Department of Chemistry, University of Cambridge, ²European Research Institute for the Biology of Aging, University Medical Centre Groningen

Summary

We describe protocols for using the wide field-of-view nematode tracking platform (WF-NTP), which enables high-throughput phenotypic characterization of large populations of Caenorhabditis elegans. These protocols can be used to characterize subtle behavioral changes in mutant strains or in response to pharmacological treatment in a highly scalable fashion.

Explore More Videos

Automated Behavioral Analysis

Wide Field of view Tracking

Sample Screening

Quantitative Assessment

Misfolding Diseases

Genetic Screenings

Nematode Growth Factor

Larvae Quantification

Parallel Worm Tracker

Imaging Software

Temperature Induction

Chapters in this video

0:04

Title

1:05

Wide Field-of-View Nematode Tracking Platform (WF-NTP) Procedure

3:40

Video Data Analysis

5:52

Results: Representative Applications Enabled by the WF-NTP Method

6:31

Conclusion

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