Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

5.4K Views

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08:12 min

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March 14th, 2019

DOI :

10.3791/58615-v

March 14th, 2019

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Matteo Canale¹, Giorgia Marisi¹, Alessandro Passardi², Emanuela Scarpi³, Paola Ulivi¹

¹Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, ²Department of Medical Oncology, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, ³Unit of Biostatistics and Clinical Trials, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS

Summary

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Keywords Circulating Biomarkers

Metastatic Colorectal Cancer

MicroRNA Panel

Translational Research

Therapy Monitoring

Molecular Characterization

Phenol chloroform Extraction

Ethanol Precipitation

Filter Cartridges

MicroRNA Purification

Chapters in this video

0:04

Title

0:57

Extraction of Circulating miRNAs from Plasma Samples

3:22

Perform Reverse Transcription and miRNA Pre-amplification

5:28

Perform Real-time PCR

6:05

Results: Increase in hsa-miR-155-5p Associated with Shorter PFS and OS

7:10

Conclusion

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