JoVE Journal
Genetics
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Nanyang Technological UniversityRetrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens
9.3K Views
•
12:24 min
•
May 5th, 2018
DOI :
May 5th, 2018
•Chapters in this video
0:04
Title
0:39
3' Barcoded Adapter Ligations with 18 Individual RNA Samples
2:00
Ligated Small RNA Purification
5:26
5' Ligated and 3' Barcoded Purified Small RNAs Reverse Transcription
8:12
Pilot PCR and Large-scale PCR Amplification
10:46
Results: Protocol Robust Whether Fresh, Frozen, or Aged
12:01
Conclusion
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ISSN 2578-6326
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