A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model

8.7K Views

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08:14 min

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February 28th, 2018

DOI :

10.3791/57182-v

February 28th, 2018

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Andrew K. Fuller¹^,², Benjamin D. Bice¹^,², Ashlee R. Venancio¹^,², Olivia M. Crowley¹^,², Ambur M. Staab¹^,², Stephanie J. Georges¹^,², Julio R. Hidalgo¹^,², Annika V. Warncke¹^,², Melinda L. Angus-Hill¹^,²

¹Division of Gastroenterology, Hepatology, and Nutrition, Department of Internal Medicine, University of Utah, ²Huntsman Cancer Institute, University of Utah

Summary

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Keywords Environmental Enrichment

Colon Microbiome

Colon Tumor Model

Microbiome Normalization

Experimental Reproducibility

Animal Stress Reduction

Breeding

Pup Rearing

Stool Collection

Chapters in this video

0:04

Title

0:50

Environmentally Enriched Cage Set Up

2:36

Stool Collection at 16 Weeks of Age

3:53

Processing gDNA from the Stool

6:00

Results: The Effect of Enhanced Environments on Microbiome Biodiversity

7:28

Conclusion

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