Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)

10.2K Views

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07:27 min

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November 1st, 2017

DOI :

10.3791/56343-v

November 1st, 2017

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Manuel Bedrossian¹, Casey Barr², Chris A. Lindensmith³, Kenneth Nealson², Jay L. Nadeau¹

¹Department of Medical Engineering, California Institute of Technology, ²Department of Earth Sciences, University of Southern California, ³Jet Propulsion Laboratory, California Institute of Technology

Summary

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Keywords Digital Holographic Microscopy

Three dimensional Imaging

Pathogen Detection

Blood Stream Infection

Cerebrospinal Fluid Infection

Petroff Hausser Counting Chamber

Live And Dead Cell Enumeration

Serial Dilution

Colony forming Units

Lysogeny Broth Media

Chapters in this video

0:05

Title

0:56

Growth and Enumeration of Bacteria

2:38

Preparation of Highly Dilute Samples for DHM and Recording DHM Videos

4:06

Calculations of Cell Density and Limits of Detection

5:22

Results: Growth Curves and Representative DHM Data

6:14

Conclusion

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