In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

17.4K Views

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08:40 min

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November 22nd, 2017

DOI :

10.3791/56301-v

November 22nd, 2017

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Evandro F. Fang¹^,⁶, Konstantinos Palikaras², Nuo Sun³, Elayne M. Fivenson¹, Ryan D. Spangler⁴, Jesse S. Kerr¹, Stephanie A. Cordonnier¹, Yujun Hou¹, Eszter Dombi⁵, Henok Kassahun⁶, Nektarios Tavernarakis²^,⁷, Joanna Poulton⁵, Hilde Nilsen⁶, Vilhelm A. Bohr¹^,⁸

¹Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, ²Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology - Hellas, ³Center for Molecular Medicine, National Heart Lung and Blood Institute, National Institutes of Health, ⁴Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, ⁵Nuffield Department of Obstetrics and Gynaecology, University of Oxford, ⁶Department of Clinical Molecular Biology, University of Oslo and Akershus University Hospital, ⁷Department of Basic Sciences, Faculty of Medicine, University of Crete, ⁸Danish Center for Healthy Aging, University of Copenhagen

Summary

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Chapters in this video

0:05

Title

1:30

Detection of Mitophagy in Human Cells using mt-Keima Plasmid

3:12

Assessing Mitophagy using Co-localization Between DCT-1 and LGG-1 in C. elegans

5:36

Detection of Mitophagy in Mice

6:19

Results: Evaluation of Mitophagy in Human Cells, C. elegans, and Mice

7:57

Conclusion

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