Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies

8.9K Views

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09:04 min

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April 22nd, 2017

DOI :

10.3791/55621-v

April 22nd, 2017

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Junning Yang¹, Chuanshen Wu², Ioana Stefanescu¹, Arie Horowitz¹^,³

¹Department of Medicine, Cardeza Vascular Research Center, Sidney Kimmel Medical College, Thomas Jefferson University, ²Department of Molecular Cardiology, Cleveland Clinic Foundation, ³Department of Cancer Biology, Cardeza Vascular Research Center, Sidney Kimmel Medical College, Thomas Jefferson University

Summary

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Keywords Embryonic Stem Cells

Neural Differentiation

Fibronectin coated Coverslips

Protein Secretion Analysis

Centrifugal Filters

Chapters in this video

0:05

Title

0:51

Embryoid Body Formation

1:58

2D Embryoid Body Culture and Detection of Secreted Proteins

3:04

3D Embryoid Body Culture

4:06

Embryoid Body Dissociation

5:56

Analysis of Embryoid Body Formation by Immunofluorescence

7:12

Results: Characteristics of Embryo Body Cultures and Visualization of Neural Differentiation Markers

8:22

Conclusion

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