Rapid Deletion Production in Fungi via Agrobacterium Mediated Transformation of OSCAR Deletion Constructs
9.9K Views
•
11:24 min
•
June 12th, 2017
DOI :
June 12th, 2017
•Chapters in this video
0:05
Title
1:02
Primer Design for PCR Amplification of Gene Flanks
2:49
Production of OSCAR Constructs
5:48
Agrobacterium tumefaciens Mediated Transformation (ATMT) of Fungi
7:45
Deletion Mutant Identification by PCR
8:53
Results: The OSCAR Method Generates a Gene Deletion Plasmid for Fungi in a Single Reaction
10:23
Conclusion
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