Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

12.2K Views

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08:58 min

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January 5th, 2017

DOI :

10.3791/55095-v

January 5th, 2017

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Hilda Vargas Robles¹, Karla Fabiola Castro Ochoa¹, Porfirio Nava², Angélica Silva Olivares³, Mineko Shibayama³, Michael Schnoor¹

¹Department of Molecular Biomedicine, Center for Research and Advanced Studies of the National Polytechnic Institute, ²Department of Physiology, Biophysics and Neurosciences, Center for Research and Advanced Studies of the National Polytechnic Institute, ³Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies of the National Polytechnic Institute

Summary

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Keywords Intestinal Epithelial Barrier

Colitis

Dextran Sulfate Sodium

Nutritional Supplements

Oxidative Stress

Neutrophil Recruitment

Gavage

Body Weight

Guaiac Fecal Occult Blood Test

Stool Consistency

Intestinal Epithelial Permeability

Evans Blue Solution

Chapters in this video

0:05

Title

0:57

Nutritional Supplement Application by Gavage and Disease Activity Index (DAI) Determination

2:14

Evans Blue Assay

3:41

Tissue Collection, Colon Histology Staining Preparation, and Oxidative Stress Analysis

6:10

Results: Representative Nutritional Supplements Effects on Intestinal Epithelial Barrier Function During Experimental Colitis

7:59

Conclusion

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