Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells

10.9K Views

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08:42 min

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January 15th, 2018

DOI :

10.3791/55063-v

January 15th, 2018

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Arash Nanbakhsh¹, Brad Best², Matthew Riese³, Sridhar Rao⁴, Li Wang⁵, Jeffrey Medin⁶, Monica S. Thakar⁶, Subramaniam Malarkannan¹^,⁵^,⁶^,⁷

¹Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute, The Blood Center of Wisconsin, ²Vector Core Lab, Blood Research Institute, The Blood Center of Wisconsin, ³Laboratory of Lymphocyte Biology, Blood Research Institute, The Blood Center of Wisconsin, ⁴Laboratory of Stem Cell Transcriptional Regulation, Blood Research Institute, The Blood Center of Wisconsin, ⁵Department of Microbiology and Immunology, The Medical College of Wisconsin, ⁶Department of Pediatrics, The Medical College of Wisconsin, ⁷Department of Medicine, The Medical College of Wisconsin

Summary

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Keywords Lentiviral Transduction

Chapters in this video

0:05

Title

0:44

Purification and Expansion of Murine Primary NK Cells

2:03

Purification and Expansion of Human Primary NK Cells

3:23

Transduction of Murine and Human Primary NK Cells with Lentivirus

6:25

Results: Lentiviral Transduction Efficiency Enhanced with Dextran

7:58

Conclusion

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