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Nanyang Technological University

High-resolution Respirometry to Assess Mitochondrial Function in Permeabilized and Intact Cells

High-resolution respirometry is used to determine mitochondrial oxygen consumption. This is a straightforward technique to determine mitochondrial respiratory chain complexes' (I-IV) respiratory rates, maximal mitochondrial electron transport system capacity, and mitochondrial outer membrane integrity.

Chapters in this video

0:05

Title

0:51

High Resolution Respirometry and Intact Cell Digitonin Titration

2:13

Mitochondrial Outer Membrane Integrity Evaluation

3:03

Measurement of ADP-stimulated and Intact Cellular Respiration

5:11

Results: Representative HepG2 Cellular Respiration Analyses

6:47

Conclusion

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