Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b

8.5K Views

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10:20 min

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November 11th, 2016

DOI :

10.3791/54540-v

November 11th, 2016

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Ye Han*¹, Kyle A. Lyman*¹, Matt Clutter², Gary E. Schiltz³, Quratul-Ain Ismail¹, Xiangying Cheng¹, Chi-Hao Luan⁴, Dane M. Chetkovich¹^,⁵

¹Davee Department of Neurology and Clinical Neurosciences, Feinberg School of Medicine, Northwestern University, ²Center for Molecular Innovation and Drug Discovery, Northwestern University, ³Department of Pharmacology, Feinberg School of Medicine, Northwestern University, ⁴High Throughput Analysis Laboratory, Department of Molecular Biosciences, Northwestern University, ⁵Department of Physiology, Feinberg School of Medicine, Northwestern University

Summary

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Keywords Small Molecule Inhibitors

Protein protein Interaction

HCN1

TRIP8b

High throughput Screening

Fluorescence Polarization

Inhibition Assay

Depression

Therapeutic Target

Acoustic Liquid Handler

384 well Plate

FITC labeled HCN1

TAMRA labeled HCN1

Chapters in this video

0:05

Title

1:08

High Throughput Screen

3:07

Confirmation of Hits Using TAMRA-tagged HCN1 Peptide

4:33

Dose-response Validation of Bead-based Proximity Assay

7:15

Results: Identification of Small Molecule Inhibitors Between HCN1 and TRIPb8 Interactions

9:14

Conclusion

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