JoVE Journal

Medicine

You have full access to this content through

Nanyang Technological University

Imaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids

12.9K Views

•

10:44 min

•

December 28th, 2015

DOI :

10.3791/53486-v

December 28th, 2015

•

Kimberley A. Beaumont¹^,², Andrea Anfosso¹^,², Farzana Ahmed³, Wolfgang Weninger*¹^,⁴^,⁵, Nikolas K. Haass*¹^,³^,⁵

¹The Centenary Institute, ²Sydney Medical School, University of Sydney, ³The University of Queensland Diamantina Institute, Translational Research Institute, The University of Queensland, ⁴Department of Dermatology, Royal Prince Alfred Hospital, ⁵Discipline of Dermatology, University of Sydney

Summary

We describe two complementary methods using the fluorescence ubiquitination cell cycle indicator (FUCCI) and image analysis or flow cytometry to identify and isolate cells in the inner G1 arrested and outer proliferating regions of 3D spheroids.

Explore More Videos

3D Tumor Spheroids

Tumor Microenvironment

Drug Penetration

Cell cell Interaction

Chapters in this video

0:05

Title

1:06

3D Spheroid Formation

3:30

Image Analysis of FUCCI Spheroid Sections

8:40

Results: Quantification of G1 Arrested and Proliferating Cells in Cancer Cell Spheroids

10:00

Conclusion

Related Videos

article

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma

19.1K Views

article

Evaluation of Cancer Stem Cell Migration Using Compartmentalizing Microfluidic Devices and Live Cell Imaging

25.2K Views

article

Modeling and Imaging 3-Dimensional Collective Cell Invasion

16.7K Views

article

Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting

18.0K Views

article

3-Dimensional Resin Casting and Imaging of Mouse Portal Vein or Intrahepatic Bile Duct System

17.1K Views

article

Patient Derived Cell Culture and Isolation of CD133⁺ Putative Cancer Stem Cells from Melanoma

21.2K Views

article

High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum

16.2K Views

article

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke

15.0K Views

article

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation

14.1K Views

article

Flow Cytometry Analysis of Immune Cell Subsets within the Murine Spleen, Bone Marrow, Lymph Nodes and Synovial Tissue in an Osteoarthritis Model

19.3K Views

Copyright © 2025 MyJoVE Corporation. All rights reserved

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More