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Bioengineering

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Nanyang Technological University

Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts

11.4K Views

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13:16 min

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December 22nd, 2015

DOI :

10.3791/53459-v

December 22nd, 2015

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Doron Cohn Yakubovich¹, Wafa Tawackoli²^,³^,⁴, Dmitriy Sheyn²^,³, Ilan Kallai¹, Xiaoyu Da⁴, Gadi Pelled¹^,²^,³^,⁴, Dan Gazit¹^,²^,³^,⁴, Zulma Gazit¹^,²^,³

¹Skeletal Biotech Laboratory, The Hebrew University–Hadassah Faculty of Dental Medicine, ²Department of Surgery, Cedars-Sinai Medical Center, ³Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, ⁴Biomedical Imaging Research Institute, Cedars-Sinai Medical Center

Summary

Implantation of autologous and allogeneic bone grafts constitute accepted approaches to treat major craniofacial bone loss. Yet the effect of graft composition on the interplay among neovascularization, cell differentiation and bone formation is unclear. We present a multimodal imaging protocol aimed to elucidate the angiogenesis-osteogenesis interdependence at the graft proximity.

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Keywords Computed Tomography

Optical Imaging

Osteogenesis angiogenesis Coupling

Bone Autografts

Bone Allografts

Vascularization

Fluorescence Imaging

Bioluminescence Imaging

Bone Regeneration

Stem Cell Recruitment

Arteriole Formation

Bone Mineralization

Chapters in this video

0:05

Title

0:54

Preparation of Bone Allografts

2:38

Calvarial Defect Surgery

5:32

Micro-computed Tomography for Characterization of the Vascular Tree

10:50

Results: Cranial Bone Autograft and Allograft Analysis

12:32

Conclusion

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