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Developmental Biology

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Nanyang Technological University

Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract

16.9K Views

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08:40 min

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December 3rd, 2015

DOI :

10.3791/53414-v

December 3rd, 2015

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Nicholas Cormier*¹, Alexander Yeo*¹, Elizabeth Fiorentino¹, Julia Paxson¹

¹Department of Biology, College of the Holy Cross

Summary

The capacity to migrate is a key function of many different cell types, including mesenchymal stromal cells (MSCs). However, quantifying alterations in migratory capacity after damage is challenging. This protocol describes an easily adaptable migration assay that uses rigorous statistics to quantify changes in MSC migratory capacity after damage.

Explore More Videos

Optimized Wound Scratch Assay

Mesenchymal Stromal Cell Migration

Quantitative Migration Analysis

Soluble Cigarette Smoke Extract

Cell Migration Assay

Scratch Assay Optimization

Cell Exclusion Zone Assay

Cell Migration Quantification

Reproducibility

Customizability

Cost effectiveness

Chapters in this video

0:05

Title

0:40

Creating the Scratch

2:51

Taking Initial Images of the Scratch

4:34

Analyzing Initial Scratch Images

6:07

Taking Final Images of the Scratch and Analyzing Final Scratch Images

6:52

Results: Migratory of Murine Lung MSCs is Altered After Damage with Cigarette Smoke Extract

8:02

Conclusion

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