JoVE Journal
Neuroscience
You have full access to this content through
Nanyang Technological UniversityConstruction of Cell-based Neurotransmitter Fluorescent Engineered Reporters (CNiFERs) for Optical Detection of Neurotransmitters In Vivo
13.2K Views
•
12:48 min
•
May 12th, 2016
DOI :
May 12th, 2016
•Chapters in this video
0:05
Title
0:56
Lentiviral Transduction of HEK293/TN-XXL/Gqi5 Cells
2:49
Preparation for FACS and Isolation of the Single CNiFER Clones
5:17
CNiFER Implantation into Mouse Cortex
7:52
In Vivo Imaging of CNiFER Clones
10:42
Results: Examples of Flexstation Analysis, Agonist-induced FRET Response and Dose Response Curves for D2 CNiFER
12:02
Conclusion
Related Videos
In vitro and in vivo Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cells
21.8K Views
Spectral Confocal Imaging of Fluorescently tagged Nicotinic Receptors in Knock-in Mice with Chronic Nicotine Administration
13.4K Views
Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo
19.9K Views
An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons
6.7K Views
TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis
10.8K Views
In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin
12.3K Views
In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters
8.3K Views
Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis
2.6K Views
Expanding the Toolkit for In Vivo Imaging of Axonal Transport
3.4K Views
Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
9.2K Views
ISSN 1940-087X
Copyright © 2025 MyJoVE Corporation. All rights reserved
We use cookies to enhance your experience on our website.
By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.