JoVE Journal

Medicine

This content is Open Access.

The Mouse Round-window Approach for Ototoxic Agent Delivery: A Rapid and Reliable Technique for Inducing Cochlear Cell Degeneration

17.2K Views

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12:21 min

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November 26th, 2015

DOI :

10.3791/53131-v

November 26th, 2015

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Shawn M. Stevens¹, LaShardai N. Brown², Paula C. Ezell³, Hainan Lang²

¹Department of Otolaryngology Head and Neck Surgery, Medical University of South Carolina, ²Department of Pathology and Laboratory Sciences, Medical University of South Carolina, ³Intuitive Surgical, Inc

Summary

Various methods exist for introducing ototoxic agents to the cochleae of animal models. Presented is a surgical protocol for delivery of ototoxic agents to the round window niche. The procedure is reliable, creates targeted intra-cochlear lesions, and avoids mechanical damage to the microarchitecture. Examination of cochlear self-repair/regeneration is possible.

Explore More Videos

Cochlear Cell Degeneration

Ototoxic Agent Delivery

Round Window Niche

Cochlear Injury

Auditory Pathophysiology

Cochlear Cell Regeneration

Chapters in this video

0:05

Title

2:10

Surgical Preparation and Positioning

5:09

Surgical Procedure

8:07

Round Window Application of Ototoxic Agent

9:52

Results: Targeted Cochlear Damage After Heptanol Exposure

11:18

Conclusion

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