JoVE Journal

Medicine

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Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy

11.6K Views

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08:09 min

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April 6th, 2015

DOI :

10.3791/52462-v

April 6th, 2015

•

Peter Bannas*¹, Alexander Lenz*¹^,², Valentin Kunick¹^,², William Fumey¹^,², Björn Rissiek², Joanna Schmid¹^,², Friedrich Haag², Axel Leingärtner³, Martin Trepel⁴, Gerhard Adam¹, Friedrich Koch-Nolte²

¹Department of Diagnostic and Interventional Radiology, University Medical Center, Hamburg, ²Institute of Immunology, University Medical Center, Hamburg, ³University Cancer Center Hamburg, University Medical Center, Hamburg, ⁴Department of Oncology and Hematology, University Medical Center, Hamburg

Summary

This protocol outlines the steps required to perform ex vivo validation of in vivo near-infrared fluorescence xenograft imaging experiments in mice using fluorophore labelled nanobodies and conventional antibodies.

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Tumor Xenograft

Subcutaneous Tumor

Antigen negative

Antigen positive

Non invasive Imaging

Ex Vivo Validation

Chapters in this video

0:05

Title

2:11

In Vivo Imaging

3:07

Harvesting and Preparation of Tumors

4:19

FACS Analysis

6:01

Results: Nanobody and mAb Staining of Tumors

7:09

Conclusion

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