Measuring the Osmotic Water Permeability Coefficient (Pf) of Spherical Cells: Isolated Plant Protoplasts as an Example
16.0K Views
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14:20 min
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October 8th, 2014
DOI :
October 8th, 2014
•Chapters in this video
0:05
Title
2:10
Isolation of Arabidopsis Mesophyll Protoplasts
4:13
The Hypotonic Challenge Assay: Arabidopsis Mesophyll Cell Swelling
6:36
Analysis of Cell Volume Change Using ImageJ
8:12
Modling the Rate of Osmolarity Change in the Experimental Chamber Using ImageJ and the Matlab Program PfFit
11:11
Determining the Pf Using the Matlab Fitting Program PfFit
12:30
Results: Determination of the Osmotic Water Permeability Coefficient (Pf) in Plant Protoplasts
13:37
Conclusion
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