JoVE Journal
Engineering
This content is Open Access.
Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
17.9K Views
•
10:03 min
•
June 27th, 2014
DOI :
June 27th, 2014
•Chapters in this video
0:05
Title
2:13
Preparation of Attenuated Total Reflection Experiments on Bacteriorhodopsin
3:48
Performing Transmission Experiments on Detergent-solubilized Channelrhodopsin-2
5:02
Adjustment and Synchronization of the Exciting Laser
5:55
Time-resolved Step-scan Preparations and Settings
7:48
Results: Time-resolved Step-scan FTIR Experiments to Determine Protonation and Protein Conformational Changes
9:24
Conclusion
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