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A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

7.7K Views

15:28 min

January 17th, 2014

DOI :

10.3791/51114-v

January 17th, 2014


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Tuberculosis

Chapters in this video

0:05

Title

1:44

Preparation of the Bacterial Suspension

3:07

High-throughput Genome-wide siRNA Screening

6:16

High-throughput Compound Screening

8:27

Image Acquisition and Analysis

11:10

Results: Mtb Colonization in Host Cells is Inhibited by Silencing Dectin-1 and by Isonazid or Rifampicin Treatment

14:37

Conclusion

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