Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition

58.6K Views

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08:01 min

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December 22nd, 2011

DOI :

10.3791/3227-v

December 22nd, 2011

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David S. Booth¹, Agustin Avila-Sakar², Yifan Cheng²

¹Graduate Group in Biophysics, University of California San Francisco , ²Department of Biochemistry and Biophysics, University of California San Francisco

Summary

Chapters in this video

0:05

Title

1:16

Making Carbon Coated EM Grids for Negative Staining EM

2:28

Carbon Coating Grids

4:27

Preparing Negative Stain EM Grids

5:57

Preparing an Electron Microscope

7:07

Electron Microscopy Images

7:30

Conclusion

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