Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae

14.6K Views

•

09:40 min

•

September 23rd, 2011

DOI :

September 23rd, 2011

•

Lauren Liddell¹^,², Glenn Manthey², Nicholas Pannunzio³, Adam Bailis²

¹Irell & Manella Graduate School of Biological Sciences, ²Department of Molecular and Cellular Biology, City of Hope Comprehensive Cancer Center and Beckman Research Institute, ³Department of Biochemistry and Molecular Biology, University of Southern California, Norris Comprehensive Cancer Center

Summary

Chapters in this video

0:05

Title

1:16

Determining HO-stimulated Translocation Frequencies

2:40

Determining Plating Efficiencies

4:05

Southern Blot Analysis

4:57

Chromosome Blot Analysis

6:13

Quantitation and Analysis of Chromosomal Translocations

9:21

Conclusion

Related Videos

article

Dissection of Saccharomyces Cerevisiae Asci

18.5K Views

article

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae

10.0K Views

article

Quantitation of γH2AX Foci in Tissue Samples

14.3K Views

article

Microarray Analysis for Saccharomyces cerevisiae

13.5K Views

article

A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae

25.4K Views

article

Expression and Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein in Saccharomyces cerevisiae

18.5K Views

article

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

34.7K Views

article

Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae

10.2K Views

article

Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein Expressed in Saccharomyces cerevisiae

14.4K Views

article

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1

13.4K Views

Copyright © 2025 MyJoVE Corporation. All rights reserved