JoVE Logo

Sign In

ConceptExperiment

You have full access to this content through

Nanyang Technological University

Preparation of Collagen-Coated Compartmented Culture Dishes for Neuronal Cell Culture

-- views • 1:23 min

Transcript

Start by adding a collagen solution to a culture dish.

The solution's acidic pH prevents collagen matrix formation.

Remove the excess solution.

Place the dish in a tray along with a gauze pad soaked in ammonium hydroxide. Place a lid to contain ammonia fumes.

These vapors raise the pH, neutralizing the acidic environment.

Neutralization disrupts the electrostatic repulsion between collagen molecules, allowing them to form a stable matrix. Create horizontal scratches across the center.

Apply a non-reactive grease to the base of a compartmented chamber.

Now, attach the scratched surface of the dish to the chamber.

Flip and apply the grease to seal the central compartment.

Add media to check for leakage. Seal the leakage with grease.

This forms a three-compartment system: the central compartment for neuronal cells and the side compartments for tumors. 

Neuronal cells grow along scratches to side compartments and produce a myelin sheath on axons, facilitating the study of the tumor-cell interaction.

article

02:30

Preparation of Collagen-Coated Compartmented Culture Dishes for Neuronal Cell Culture

Related Videos

31 Views

article

08:02

Analysis of Cell Migration within a Three-dimensional Collagen Matrix

Related Videos

23.6K Views

article

10:08

Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions

Related Videos

5.8K Views

article

07:39

Co-culture of Glutamatergic Neurons and Pediatric High-Grade Glioma Cells Into Microfluidic Devices to Assess Electrical Interactions

Related Videos

3.4K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved