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Nanyang Technological University

Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast

Gene deletion and protein overexpression are common methods for studying functions of proteins. In this article, we describe a protocol for analysis of phenotype development as a function of protein concentration at population and single-cell levels in Saccharomyces cerevisiae.

Chapters in this video

0:00

Title

0:49

Introduction

1:30

Cell Culture and Protein Induction

4:00

Quantification of Cell-Division Phenotype and Cell Death

5:32

Visualizing Phenotype-Specific Defects by Fluorescence Microscopy

7:58

Determination of Phenotype Development at the Single Cell Level by Time-Lapse Video Microscopy

11:55

Effects of ENTH2 Overexpression

14:23

Conclusion

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