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Nanyang Technological University

A Practical Approach to Genetic Inducible Fate Mapping: A Visual Guide to Mark and Track Cells In Vivo

16.7K Views

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13:36 min

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December 30th, 2009

DOI :

December 30th, 2009

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Ashly Brown¹, Stephen Brown², Debra Ellisor², Nellwyn Hagan¹, Elizabeth Normand¹, Mark Zervas²

¹Department of Neuroscience, Division of Biology and Medicine, Brown University, ²Department of Molecular Biology, Cell Biology and Biochemistry, Division of Biology and Medicine, Brown University

Summary

Genetic Inducible Fate Mapping (GIFM) marks and tracks cells with fine spatial and temporal control in vivo and elucidates how cells from a specific genetic lineage contribute to developing and adult tissues. Demonstrated here are the techniques required to fate map E12.5 mouse embryos for epifluorescent and explant analysis.

Chapters in this video

0:00

Title

0:31

Introduction

2:14

Administering Tamoxifen by Oral Gavage

4:17

Whole Mount Microscopy of E12.5 Embryos

5:10

Craniotomy and Adult Whole Mount Microscopy

8:03

Explant Preparation and Micro-Dissection of the Ventral Mesencephalon from E12.5 Embryos

9:59

Representative Results for Wnt1 Fate-Mapping

12:11

Conclusion

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