Name: Video: Elektrik Stimülasyonu Kullanılarak Bir Sıçan Modelinde Akut Pons Enfarktüsünün İndüklenmesi - Kavram
Uploaded: 2025-01-30T14:42:56.000+00:00
Duration: 1 min 16 s
Description: 31 Görüntüleme. Kaynak: Luo, M., et al. Elektrik Stimülasyonu ile Sıçanlarda Akut Pontin Enfarktüsünün Kurulması. J. Vis. Uzm. (2020) Bu video, elektrik stimülasyonu kullanarak anestezi uygulanmış bir sıçanın ponsunda doku hasarını veya enfarktüsünü indüklemek için bir prosedürü göstermektedir.

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All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Establishment of infarction in the pons
<ol>
	<li>Preheat the heating pad immediately before anesthesia.</li>
	<li>Attach the skull drill to the holder on the stereotaxic frame.</li>
	<li>Intraperitoneally inject the rats with 50 mg/kg ketamine and 5 mg/kg xylazine. Check for the lack of toe-pinch response.</li>
	<li>Mount the rat onto the stereotaxic frame in the prone position. Position the ear bars above the ear canal to secure the head. Ensure that the skull is kept horizontal to avoid any skewing of the injection.</li>
	<li>Maintain anesthesia by isoflurane (100% oxygen, 2.5% isoflurane) via a stereotaxic nose cone attachment for rats with inlet and outlet ports. Use a heating pad to maintain the temperature at 37 &#176;C and monitor it throughout the procedure.</li>
	<li>Use eye ointment to prevent corneal drying. Use forceps to pinch the paws to ensure no pain response slightly.</li>
	<li>Shave the hair of the skull with a micro-shaver. Circularly apply chlorhexidine surgical scrub starting at the surgical incision site and rotating outward.</li>
	<li>Make a 3 cm midline incision with a scalpel from the line of the bilateral lateral canthus to 0.5 cm behind the posterior fontanelle, which a surgical marker pen should mark.</li>
	<li>Use a cotton swab to remove blood.</li>
	<li>A piece of surgical tape should be placed on each side of the skin flap to expose the scalp (Figure 1).</li>
	<li>Gently remove the connective tissues from the skull bone with a cotton swab dipped in 0.9% NaCl. If not removed, the connective tissues will get caught in the drill.</li>
	<li>Identify the bregma. Choose the central point of the bregma as the origin point and mark it using a fine-tip black surgical marker pen.</li>
	<li>Place a drill at 6.0 mm AP, 2.0 mm ML (range from 0.5&#8211;3.0 mm, Figure 2A).</li>
	<li>Perform craniotomy (1 mm diameter) using an automatic drill. Proceed carefully because this point is close to the venous sinus.</li>
	<li>Remove the drill from the stereotaxic frame.</li>
	<li>Place a 22 G probe with an insulated sheath in the stereotaxic frame (Figure 3A). The probe tip should be placed 2 mm above the proximal end of the sheath (Figure 3A, B; Figure 2B).</li>
	<li>Ensure that the sheath enters the brain 7 mm (7 mm DV, Figure 2B; Figure 1C).</li>
	<li>Advance the probe along the sheath (Figure 1D) until the tip of the probe is 9 mm below the surface of the brain (Figure 2D).</li>
	<li>Connect the electrodes to an electrical stimulator (Figure 3C). Connect the anode to the probe as shown in Figure 1D. Connect the cathode to the rats (usually to the ear of the rats).</li>
	<li>Turn the electrical stimulator on and set up the following parameters: single pulse width = 4,050 ms; voltage = 50 V; and current = 4 mA (Figure 3C). During electrical stimulation, the rat will exhibit trembling. In this study, the device was not turned on for control group rats used for the behavioral tests, MRI, and TTC.</li>
	<li>Leave the probe in position for 5 min after stimulation.</li>
	<li>Remove the probe from the brain (Figure 1F).</li>
	<li>Use bone cement to cover the craniotomy. Allow the cement to dry before wound suturing.</li>
	<li>Suture the wound with 4-0 polyamide suture filaments. After three or four stiches, tie 2-1-1 standard surgical knots.</li>
	<li>Inject the rats with penicillin (0.25 mL, 80 IU diluted in 4 mL of saline) intraperitoneally to prevent infection.</li>
	<li>Inject the rats subcutaneously with meloxicam at a dose of 2 mg/kg and then repeat it every 24 h.</li>
	<li>Monitor the rats every 15 min until fully awake and return them to the cage with a heating pad. Provide free access to food and water until sacrifice. 
	NOTE: All procedures should follow the aseptic surgical principles. Before the surgery, put on a scrub top, surgical mask, and sterile gloves after a surgical scrub of the hands. Always maintain sterile suture material within the sterile field.</li>
</ol>

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>4-0 sucture</td>
			<td>Shanghai Jinzhong</td>
			<td></td>
			<td>Surgical instruments</td>
		</tr>
		<tr>
			<td>Adhesive tape</td>
			<td>Shanghai Jinzhong</td>
			<td></td>
			<td>Surgical instruments</td>
		</tr>
		<tr>
			<td>Animal anesthesia system</td>
			<td>RWD</td>
			<td></td>
			<td>Wear mask when using the system</td>
		</tr>
		<tr>
			<td>Bone cement</td>
			<td>Shanghai Jinzhong</td>
			<td></td>
			<td>Surgical instrument</td>
		</tr>
		<tr>
			<td>Cured clamp</td>
			<td>Shanghai Jinzhong</td>
			<td></td>
			<td>Surgical instrument</td>
		</tr>
		<tr>
			<td>General tissue scissors</td>
			<td>Shanghai Jinzhong</td>
			<td></td>
			<td>Surgical instrument</td>
		</tr>
		<tr>
			<td>IndoPhors</td>
			<td>Guoyao of China</td>
			<td></td>
			<td>Sterilization</td>
		</tr>
		<tr>
			<td>Isoflurane</td>
			<td>RWD</td>
			<td>217181101</td>
			<td></td>
		</tr>
		<tr>
			<td>Lesion Making Device</td>
			<td>Shanghai Yuyan</td>
			<td></td>
			<td>Making a lesion</td>
		</tr>
		<tr>
			<td>MRI system</td>
			<td>Bruker Biospin</td>
			<td></td>
			<td>Confirmation of infarction in vivo</td>
		</tr>
		<tr>
			<td>Needle holder</td>
			<td>Shanghai Jinzhong</td>
			<td></td>
			<td>Surgical instrument</td>
		</tr>
		<tr>
			<td>Penicilin</td>
			<td>Guoyao of China</td>
			<td></td>
			<td>Infection Prevention</td>
		</tr>
		<tr>
			<td>Probe</td>
			<td>Anke</td>
			<td></td>
			<td>Need some modification</td>
		</tr>
		<tr>
			<td>Q-tips</td>
			<td>Shanghai Jinzhong</td>
			<td></td>
			<td>Surgical instrument</td>
		</tr>
		<tr>
			<td>Shearing scissors</td>
			<td>Shanghai Jinzhong</td>
			<td></td>
			<td>Surgical instrument</td>
		</tr>
		<tr>
			<td>Stereotaxic apparatus</td>
			<td>RWD</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Suture needle</td>
			<td>Shanghai Jinzhong</td>
			<td></td>
			<td>Surgical instrument</td>
		</tr>
		<tr>
			<td>Tissue holding forcepts</td>
			<td>Shanghai Jinzhong</td>
			<td></td>
			<td>Surgical instrument</td>
		</tr>
		<tr>
			<td>TTC</td>
			<td>Sigma-Aldrich</td>
			<td>BCBW5177</td>
			<td>For infarction confirmation in vitro</td>
		</tr>
	</tbody>
</table>

inducing acute pons infarction in a rat model using electrical stimulation

Source: Luo, M., et al. <a href="https://app-jove-com.remotexs.ntu.edu.sg/v/60783">Establishment of Acute Pontine Infarction in Rats by Electrical Stimulation.</a> J. Vis. Exp. (2020)
This video demonstrates a procedure to induce tissue damage or infarction in the pons of an anesthetized rat using electrical stimulation.

<img alt="Figure 1" src="/files/ftp_upload/23001/23001_Figure1.jpg" /> 
Figure 1: Infarction establishment. (A) A hole made in the skull. (B) The sheath is moved to the hole. (C) Injection of the sheath. (D) Injection of the probe. (E) The anode (red arrow) is connected. (F) The probe is removed. (G) Hole (red arrow) left in the brain surface.
<img alt="Figure 2" src="/files/ftp_upload/23001/23001_Figure2.jpg" /> 
Figure 2: Location of probe. (A) Schematic diagram of stereotaxic locations: arrows point to retraction of skin flaps, site of Bregma, and positioning of drill. (B) Schematic diagram of the sheath and probe. (C) Location of tip of sheath placed in the pons. (D) Location of tip of the probe placed in the pons. (E) Experimental design.
<img alt="Figure 3" src="/files/ftp_upload/23001/23001_Figure3.jpg" /> 
Figure 3: Lesion-producing device. (A) Separate of sheath and probe. (B) The probe in the sheath. (C) The blue electrode was anode which was connected to the caudal probe; the red electrode was cathode. (D) Electrical stimulator. (E) Surgical instruments.

Inducing Acute Pons Infarction in a Rat Model Using Electrical Stimulation

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. ...

Secure an anesthetized rat in a stereotaxic unit.
Remove the fur from the scalp and sanitize the incision site. Make a midline incision and retract the skin to expose the skull.
Remove the connective tissue to avoid interference with the drill. Identify the bregma as a reference point to determine the coordinates of the pons, a structure located in the brainstem.
Drill a hole in the skull.
Insert a probe through the hole to reach the pons.
Next, connect the electrodes to an electrical stimulator, attaching the anode to the probe for brain stimulation and the cathode to the rat&#39;s ear to complete the circuit.
Deliver an electrical pulse to the pons, inducing localized tissue damage or infarction.
After stimulation, leave the probe in place briefly to stabilize the stimulation effect.
Then, slowly remove the probe and seal the surgical site with bone cement.
Suture the incision and return the rat to its cage for recovery and neurological analysis.

inducing-acute-pons-infarction-rat-model-using-electrical

Nanyang Technological University

Research

JoVE Encyclopedia of Experiments

Neuroscience

Neuropathology

Cerebrovascular Diseases

31 Görüntüleme. Kaynak: Luo, M., et al. Elektrik Stimülasyonu ile Sıçanlarda Akut Pontin Enfarktüsünün Kurulması. J. Vis. Uzm. (2020) Bu video, elektrik stimülasyonu kullanarak anestezi uygulanmış bir sıçanın ponsunda doku hasarını veya enfarktüsünü indüklemek için bir prosedürü göstermektedir. 

Video: Elektrik Stimülasyonu Kullanılarak Bir Sıçan Modelinde Akut Pons Enfarktüsünün İndüklenmesi - Kavram

Minimal Invasive Surgical Procedure of Inducing Myocardial Infarction in Mice

Myocardial infarction still remains the main cause of death in western countries, despite considerable progress in the stent development area in the last decades. For clarification of the underlying mechanisms and the development of new therapeutic strategies, the availability of valid animal models are mandatory. Since we need new insights into pathomechanisms of cardiovascular diseases under in vivo conditions to combat myocardial infarction, the validity of the animal model is a crucial aspect. However, protection of animals are highly relevant in this context. Therefore, we establish a minimally invasive and simple model of myocardial infarction in mice, which assures a high reproducibility and survival rate of animals. Thus, this models fulfils the requirements of the 3R principle (Replacement, Refinement and Reduction) for animal experiments and assure the scientific information needed for further developing of therapeutical strategies for cardiovascular diseases.

A highly reproducible model for myocardial infarction in mice with minimal invasive manipulations is described. The model can be easily performed, resulting in a high reproducibility and survival rate. Thus, the described model will reduce the number of required animals as requested by the 3R principle (Replacement, Refinement and Reduction).

Farelerde Miyokard İnfarktüsü Oluşturan Minimal İnvaziv Cerrahi Prosedür

Myocardial infarction still remains the main cause of death in western countries, despite considerable progress in the stent development area in the last ...

JoVE Journal

Tıp

Myocardial Infarction in Neonatal Mice, A Model of Cardiac Regeneration

Myocardial infarction induced by coronary artery ligation has been used in many animal models as a tool to study the mechanisms of cardiac repair and regeneration, and to define new targets for therapeutics. For decades, models of complete heart regeneration existed in amphibians and fish, but a mammalian counterpart was not available. The recent discovery of a postnatal window during which mice possess regenerative capabilities has led to the establishment of a mammalian model of cardiac regeneration. A surgical model of mammalian cardiac regeneration in the neonatal mouse is presented herein. Briefly, postnatal day 1 (P1) mice are anesthetized by isoflurane and placed on an ice pad to induce hypothermia. After the chest is opened, and the left anterior descending coronary artery (LAD) is visualized, a suture is placed around the LAD to inflict myocardial ischemia in the left ventricle. The surgical procedure takes 10-15 min. Visualizing the coronary artery is crucial for accurate suture placement and reproducibility. Myocardial infarction and cardiac dysfunction are confirmed by triphenyl-tetrazolium chloride (TTC) staining and echocardiography, respectively. Complete regeneration 21 days post myocardial infarction is verified by histology. This protocol can be used to as a tool to elucidate mechanisms of mammalian cardiac regeneration after myocardial infarction.

This protocol describes a highly reproducible model of cardiac regeneration by surgical induction of myocardial infarction in the left ventricle of postnatal day 1 mice. The method involves induction of hypothermic anesthesia and ligation of the left anterior descending coronary artery.

Yenidoğan Farelerde Miyokard Enfarktüsü, Kalp Rejenerasyon Bir Model

Myocardial infarction induced by coronary artery ligation has been used in many animal models as a tool to study the mechanisms of cardiac repair and ...

Real-time Pressure-volume Analysis of Acute Myocardial Infarction in Mice

Acute myocardial infarction can lead to acute heart failure and cardiogenic shock. The evaluation of hemodynamics is critical for the evaluation of any potential therapeutic approach directed against acute left ventricular (LV) dysfunction. Current imaging modalities (e.g., echocardiography and magnetic resonance imaging) have several limitations since data on LV pressure cannot directly be measured. LV catheterization in mice undergoing coronary artery occlusion could serve as a novel method for a real-time evaluation of LV function.
At the beginning of the procedure, mice were anesthetized followed by endotracheal intubation. For LV catheterization, the right carotid artery was exposed via middle-neck incision. The catheter was introduced and placed into the LV cavity. Left thoracotomy was conducted and the left main coronary artery (LCA) was ligated. To induce reperfusion, the suture was released after 45 min. Pressure-volume data was recorded at all times.
Ligation of the LCA caused a decrease in LV systolic function as evidenced by a 30% reduction in stroke volume, LV ejection fraction (EF), and cardiac output. Maximum dP/dt as a parameter for LV contractility was also significantly reduced and diastolic function was severely impaired (minimum dP/dt -40%). Reperfusion over a period of 20 min did not lead to a complete recovery of LV function.
Real-time pressure-volume analysis served as a valid procedure for monitoring cardiac function during acute myocardial infarction in mice. Maintaining stable anesthesia and a standardized surgical approach was crucial to ensure valid results. As the early phase of acute myocardial infarction is critical for morbidity and mortality, the delineated method could be beneficial for preclinical evaluation of new strategies for cardioprotection.

Acute myocardial infarction in mice induces acute but incompletely characterized changes in left ventricular (LV) function. LV catheterization in mice undergoing coronary artery occlusion serves as a novel method for a real-time evaluation of LV function.

Akut miyokard infarktüsünde farelerde gerçek zamanlı basınç-hacim Analizi

Acute myocardial infarction can lead to acute heart failure and cardiogenic shock. The evaluation of hemodynamics is critical for the evaluation of any ...

Inducing Acute Pons Infarction in a Rat Model Using Electrical Stimulation

TRANSKRIPT

Inducing Acute Pons Infarction in a Rat Model Using Electrical Stimulation