Одноклеточный экспрессии генов профилирование с помощью FACS и КПЦР с внутренними стандартами

0:56

Preparation of Standards from Purified DNA Amplicons and Treatment of MCF7 p53-Venus Cells

1:53

Preparation of Lysis Buffer for Cell Sorting

4:15

Cell Sorter Setup, Cell Harvesting, and Sorting

5:47

Exonuclease Treatment and Sort Quality Control Using qPCR

7:02

Gene Expression Measurement Using Microfluidic qPCR

9:03

Results: Gene Expression Profiling in Single Cells Using FACS Analysis and qPCR

10:09

Conclusion