JoVE Journal
Biochemistry
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Nanyang Technological University2.1K Views
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08:48 min
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December 9th, 2020
DOI :
December 9th, 2020
•0:04
Introduction
0:53
Preparation of NE Depleted of U1 snRNP (U1ΔNE)
2:10
Immunoprecipitation of 10S Fractions of Glycerol Gradients by Anti-Gal3
3:52
Assembly of Splicing Reaction and Analysis of Products
6:09
Results: RNA and Polypeptide Compositions of the Anti-Gal3 Precipitate of the 10S Region of the Glycerol Gradient and its Ability to Reconstitute Splicing in U1ΔNE
8:08
Conclusion
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ISSN 2578-2037
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