JoVE Journal

Immunology and Infection

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Nanyang Technological University

Atacante Genetics telas usando macrófagos para Identificar

Forward genetics is a powerful approach to identify genes in intracellular pathogens important for resistance to cell autonomous immunity. The current approach uses innate immune cells, specifically macrophages, to identify novel Toxoplasma gondii genes important for immune evasion.

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Title

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Isolation of Parasite Mutants with a Fitness Defect Following Activation of Infected Macrophages

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Evaluation of Mutants to Determine if the Defect is at the Level of Parasite Survival or Replication Following Activation of Infected Macrophages

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Results: T. gondii Mutants with Fitness Defects are Identified by Phase Contrast and Immunofluorescence Microscopy

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Conclusion

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