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Medicine

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Nanyang Technological University

Preparation and Analysis of Histological Slides of Rat and Mouse Eyeballs to Evaluate the Retina

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854 Views

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07:01 min

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August 23rd, 2024

DOI :

10.3791/66663-v

August 23rd, 2024

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Ewa Sikorska*¹, Dominika Wołosz*², Kaja Kasarełło¹, Łukasz Koperski², Barbara Górnicka², Agnieszka Cudnoch-Jędrzejewska¹

¹Chair and Department of Experimental and Clinical Physiology, Laboratory of Centre for Preclinical Research, Medical University of Warsaw, ²Department of Pathology, Medical University of Warsaw

Podsumowanie

This manuscript presents a protocol for the histological preparation of slides from rodent eyeballs. With the proposed method, the inner retina can be easily visualized and assessed under a light microscope. The procedure is presented for the eyeballs of mice and rats.

Przeglądaj więcej filmów

Histological Slides

Eye Examination

Ophthalmic Diseases

Neurodegenerative Disorders

Vascular Disorders

Histopathological Examination

Morphology Assessment

Paraformaldehyde Fixation

Ethanol Dehydration

Paraffin Infiltration

Xylene Clearing

Histological Protocol

Tissue Processing

Rozdziały w tym wideo

0:00

Introduction

1:16

Preparation and Slide Mounting of Rat Eyeball Sections for Histological Analysis

4:38

Assessing Rat Eyeball Sections for Retinal Morphology Using Histopathological Techniques

6:40

Representative Results

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