JoVE Journal

Immunology and Infection

This content is Free Access.

Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test

Please note that all translations are automatically generated. Click here for the English version.

1.3K Views

•

05:25 min

•

July 14th, 2023

DOI :

10.3791/65323-v

July 14th, 2023

•

Julia Häring*¹, Tanja Michel*¹, Matthias Becker¹, Daniel Junker¹, Tatia Tchitchagua², Olaf Leschnik², Berit Lange³^,⁴, Stefanie Castell³^,⁴, Gérard Krause³^,⁴, Monika Strengert³, Alex Dulovic¹, Nicole Schneiderhan-Marra¹

¹NMI Natural and Medical Sciences Institute at the University of Tübingen, ²Department of Neurology, Sächsisches Krankenhaus Rodewisch, ³Department of Epidemiology, Helmholtz Centre for Infection Research, ⁴German Centre for Infection Research (DZIF)

Podsumowanie

A three-channel dual-reporter fluorescence flow analysis system was used to develop a bead-based multiplex immunoassay that simultaneously evaluates serum samples for IgG and IgM elicited against multiple antigens of different Borrelia species that cause Lyme borreliosis in Europe and North America.

Przeglądaj więcej filmów

Lyme Borreliosis

Antibody Classes

Multiplex Serological Test

Immunoreactivity

Dual reporter System

Luminex Intelliflex

Pathogen Identification

Infection Detection

Serosurveillance

Vaccine Development

Immune Response Characterization

Pathogenic Agents

Rozdziały w tym wideo

0:00

Introduction

1:57

Dual‐Reporter IgG and IgM Serological Assay for Simultaneous Antibody Detection of Borrelia

Powiązane Filmy

article

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

11.7K Views

article

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce

12.9K Views

article

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

14.2K Views

article

Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung

32.8K Views

article

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

28.3K Views

article

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

4.9K Views

article

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

2.7K Views

article

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

2.8K Views

article

Multipleksowa ilościowa detekcja bakterii przy użyciu mikrosfer magnetycznych znakowanych barwnikiem

100 Views

article

Multianalitowy test immunobealikowy: Technika testu immunologicznego oparta na przepływie do jednoczesnego wykrywania wielu podtypów przeciwciał w próbce ludzkiej surowicy

46 Views

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone

Używamy plików cookie, aby ulepszyć Twoje wrażenia z korzystania z naszej witryny.

Kontynuując korzystanie z naszej witryny lub klikając „Kontynuuj”, wyrażasz zgodę na akceptację naszych plików cookie.

Dowiedz się więcej