JoVE Logo

Zaloguj się

Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch

893 Views

09:10 min

April 21st, 2023

DOI :

10.3791/65122-v

April 21st, 2023


Przeglądaj więcej filmów

Saphenous Vein

Rozdziały w tym wideo

0:04

Introduction

1:18

Isolation and Culture of Primary Human Saphenous Vein Endothelial Cells (hSVECs)

3:37

Applying Shear Stress on Human SVECs

5:42

Applying Cyclic Stretch on Human SVECs

7:20

Results: Effects of Mechanical Stress in Human SVECs

8:34

Conclusion

Powiązane Filmy

article

12:43

On-Chip Endothelial Inflammatory Phenotyping

10.8K Views

article

08:54

Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions

17.1K Views

article

16:46

A Novel Stretching Platform for Applications in Cell and Tissue Mechanobiology

11.7K Views

article

11:38

Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay

3.3K Views

article

09:50

Procedure for Human Saphenous Veins Ex Vivo Perfusion and External Reinforcement

11.2K Views

article

08:50

Gene Expression Analysis of Endothelial Cells Exposed to Shear Stress Using Multiple Parallel-plate Flow Chambers

8.9K Views

article

12:28

Segmenting Growth of Endothelial Cells in 6-Well Plates on an Orbital Shaker for Mechanobiological Studies

4.9K Views

article

07:17

Electrophysiological Recordings of Single-cell Ion Currents Under Well-defined Shear Stress

6.1K Views

article

09:34

Pneumococcus Infection of Primary Human Endothelial Cells in Constant Flow

6.3K Views

article

08:53

In Vitro Model Integrating Substrate Stiffness and Flow to Study Endothelial Cell Responses

379 Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone